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Altace hct dose, 0.7 mg/kg) (Babys, 1993) (Babys) and the non-selective cyclophosphamide (50 mg/kg, subcutaneous, IV) (Hobson et al., 1985; MacLeod 1990). A dose of 0.5 mg/kg DIO was given intramuscularly in rats treated for a total of three weeks with a single dose of Dio (20 mg/kg) (Babys, 1988). Although there was no clinical effect in rats on the primary endpoint of spontaneous locomotor activity, these results were interpreted as being in some way negative, because no effect of DIO on body weight gain was detected (MacLeod et al., 1990), the animal died within 10 days from a heart failure induced by high blood pressure Clotrimazole and betamethasone dipropionate cream for sale (Babys, 1988), and DIO administration in male albino rats had no significant effect on the sexual behaviour of females (Babys, 1989). This latter study, however, was based solely on studies performed in albino males and included only male female rats, while the same authors previously reported on their studies in normal male and female rats, in which, even if they were not fully albino, the animals were in a similar sexual condition as the previously albino control rats in both tests (Babys, 1989). These findings are in keeping with results elsewhere, for example in studies of hypopituitarism male rats with the use of a rat and mouse model of hypopituitarism produced by the transgenic drug clozapine (Garcia-Avila et al., 1998). The studies described above are thus consistent with a more negative finding of DIO in male rats, as well suggesting that the mechanism involved in these effects (hypoactivation of catecholamine systems in the male rat) is likely to be the same as that for hypopituitarism in the female, though at lower doses (MacLeod et al., 1990). Animal data obtained in rats, that the mechanisms underlying beneficial effect of DIO in male animals are similar to those in female animals, also allow a comparison of effects in rats with DIO compared other drugs in which an anxiogenic effect has been reported. DIO, with or without the benzyl alcohol extract, is an effective and safe pharmacological agent for induction of anaesthesia in animals, but the mechanisms by which it produces such action are not fully understood. This drug has been reported to be effective for an initial 4–6 seconds and then after this a gradual recovery of the anaesthetic response, in terms of reduced anxiety (Baldwin, 1989; MacLeod et al., 1990; Walker, 1986; Walker & McEwen, 1988). Because of such long-lasting action, the mechanism underpinning beneficial anxiogenic activity of DIO is also relevant. Dio, with or without the benzyl alcohol extract, produces behavioural effects in rats of such duration that, in the case of rats, it is difficult but not impossible to extrapolate the results humans. However, DIO produces a marked and persistent anxiogenic reaction in rats which, when extrapolated to humans, would suggest that one cannot be certain as to which mechanism underlies this phenomenon until more long-term research is carried out (Walker & McEwen, 1988). A comparison between effects of DIO and those reported in human beings who suffer from anxiety disorders such as panic attacks (Dey, 1989; Dye et al., 1991), is difficult for several reasons. Firstly, panic attacks seem to display the characteristic feature of being episodic, occurring episodically in some individuals or only transiently in others (Dey). Anxiety disorders are more stable in individuals than the animal Ciprofloxacin 500 mg buy online species in which they originate and occur frequently, for example at the beginning of work week; they are sometimes prolonged to weeks or even months depending upon their duration in each individual. Secondly, there can be no direct comparison between the pharmacological properties of DIO and those benzyl alcohol, such as produced by the ethyl alcohol extract of B. oleracea, the ethyl benzyl ether extract of A. officinalis and the ethyl ether extract of C. alnifolia, which all appear to have similar mechanisms (Wendel & Köhler, 1989). Phenylbenzylalcohol, a chemical product of the plant B. oleracea, was previously tested by using an initial 40% DIO dose (Babys, 1988; MacLeod et al., 1990), whereas phenylbenzyl ether and DIO have similar potency, such that the total dose needed to produce equivalent levels of anxiogenic behaviour with phenylbenzyl alcohol is 0.4 mg/kg, and with DIO is about 1 g/kg (Babys, 1988). Because the animal studies (Mac)

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